AlbumiNZ™ Bovine serum albumin (BSA), MP Biomedicals

Supplier: MP Biomedicals

0219989690
ICNA0219989690EA 3340 SGD
ICNA0219989690
AlbumiNZ™ Bovine serum albumin (BSA), MP Biomedicals
Proteins and Peptides

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55 to 62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at one time with the remainder being in extracellular spaces with which there is, in general, equilibration about every 24 hours.


Albumin binds water, Ca²⁺, Na⁺, and K⁺. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Albumin is used to solubilise lipids, stabilise protein solutions, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present such as electrophoresis.


Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5 to 7 it contains 17 intrachain disulphide bridges and 1 sulphydryl group.


Biochem/physiol Actions:
Albumin loses its helical structure rapidly in 2 to 4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulphide bonds. Reduced albumin can reform into structures capable of binding antibodies and other ligands. Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist. After oxidation of the cystine bridges, however, native structure is irreversibly lost.

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SPECIFICATIONS

Protein/peptide name Bovine Serum Albumin
Environmentally Preferable
Protein synonyms BSA|Fraction V|Cohn Fraction V
Species
Source Serum
CAS No. 9048-46-8
Endotoxin level Low
Purity >97%
Grade Stem Cell Grade
Concentration ≥95%
Form Powder
Storage conditions Ambient
pH 6,5 - 7,5

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