Guanidinium thiocyanate for molecular biology
Supplier: Thermo Scientific
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Warning
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Synonyms:
Guanidinium sulphocyanide, GTC, Guanidine Thiocyanate, Guanidinium rhodanide
Guanidine thiocyanate is used for RNA isolation and can solubilise proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
Soluble in H₂O (1420 g/L) at 20°C, and ethanol (5%).
Caution: Light Sensitive. Keep container tightly closed and store away from oxidising agents.
Formula:
CH₅N₃·CHNS MW: 118,16 g/mol Melting Pt: 118…122 °C Density: 1,29 Storage Temperature: Ambient |
MDL Number:
MFCD00013027 CAS Number: 593-84-0 EINECS: 209-812-1 |
Specification Test Results
Appearance (Colour) | White to off-white |
Form | Crystalline powder |
Assay (Titration) | 99.0% min. |
DNase | None detected |
Protease activity | None detected |
RNase | None detected |
UV/Vis A280 (70%, water) | 0.8 |
UV/Vis A300 (6M, water) | 0.1 |
UV/Vis A410 (6M, water) | 0.1 |
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