HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), anhydrous ≥99.5% for biopharmaceutical production

Supplier: VWR Chemicals

VWRC30487.297EA 243 SGD
VWRC30487.297 VWRC30487.366 VWRC30487.468
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid), anhydrous ≥99.5% for biopharmaceutical production
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES

Formula: C₈H₁₈N₂O₄S
Melting Pt: 210…215 °C
Density: 1,448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
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Specification Test Results

Assay Min. 99.5 %
Appearance White crystalline powder
Cell culture toxicity Passes test
DNases Not detected
Identification by IR Conforms to structure
Proteases Not detected
RNases Not detected
Solubility (1 mol/l) Clear.colourless.complete
pKa (20°C) 7.45 - 7.65
Heavy metals (as Pb) Max. 5 ppm
Ignition residue Max. 0.3 %
Water Max. 0.5 %
Ca (Calcium) Max. 5 ppm
Cu (Copper) Max. 5 ppm
Fe (Iron) Max. 5 ppm
Mg (Magnesium) Max. 5 ppm
Mn (Manganese) Max. 5 ppm
Absorbance (260 nm) (1 mol/l) Max. 0.10
Absorbance (280 nm) (1 mol/l) Max. 0.10
Bioburden Max. 100 CFU/g
Endotoxin (0,2 %) Max. 0.1 EU/mg

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