Wheat germ lectin
Supplier: MP Biomedicals
Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. They are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required.
- Dissolvable in distilled water and 0,9% NaCl within five minutes
- Can be reconstitute with buffer to a concentration of 1 mg/ml
- Extinction coefficient (E1%): 1,09×10⁵ (Lit.)
- Carbohydrate specificity: (GlcNAc-b-(1,4)-GlcNAc)1-4> b-GleNAc>Neu5Ac
- Inhibitory carbohydrate: GlcNAcb(1,4)GlcNAcb(1,4)GlcNAc>GlcNAcb(1,4)GlcNAc>Glc sialic acid(Neu5Ac)>>GalNAc
They are very useful in a wide variety of applications in vitro, including blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, lymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.
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