Calf alkaline phosphatase (from intestine)

Supplier: MP Biomedicals

0219068001 0219068010
ICNA0219068001EA 221 SGD
ICNA0219068001 ICNA0219068010
Calf alkaline phosphatase (from intestine)
Enzymes

Alkaline phosphatase is a phosphomonoesterase, used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.


  • Enzyme Commission Number: E.C. 3.1.3.1
  • Isoelectric point (pI): 5,7 (Lit.)
  • Free of endonuclease, exonuclease, and RNase activities


It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturisation of milk. It is commonly conjugated to antibodies and used in immunoblotting, ELISA's, and other antibody based detection assays.


Activators: Zn, Mg²⁺, Ca²⁺, Tris buffer, diethanolamine
Inhibitors: Acidification to pH 4,5 reversibly inactives the enzyme; inorganic phosphate, chelating agents such as EDTA


Unit definition: That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25 °C and pH 9,6.

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