IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99%, white powder
Supplier: MP Biomedicals
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Warning
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Synonyms:
IPTG, (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol, Isopropyl Beta-D-1-thiogalactopyranoside
IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.
- Presentation: Crystalline, Dioxane-free,β-Galactosidase inducer
- Soluble in methanol and water (50 mg/mL - clear, colorless solution)
- Storage temperature: -20°C, desiccate
IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. The compound is widely used with X-Gal (5-Bromo-4- chloro-3-indolyl- beta-D-galactopyranoside, MP catalog number 150001) plates to screen for blue and white colonies. The lac operon system and IPTG have been used in multiple cloning experiments and cellular systems to study gene activity. The final concentration typically used in indicator plates is 0,2 mM.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies. (Rafal Zielinski,2012), Affibody-dylight conjugates for invivo assessment of HER2 expression by near-infrared optical imaging, Public library of science one, 7(7), 2012).
Formula:
C₉H₁₈O₅S MW: 238,31 g/mol Melting Pt: 105…115 °C Storage Temperature: Freezer |
MDL Number:
MFCD00063273 CAS Number: 367-93-1 |
Specification Test Results
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Standard |
pH (5% aq) | 5.0-7.0 |
Optical Rotation (c=1, water) | -26° to -33° |
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