3,3',5,5'-Tetramethylbenzidine (TMB) ≥99%, powder

Supplier: MP Biomedicals
Warning

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

02190277.1 0219027701 0219027705
ICNA02190277.1EA 77.3 SGD
ICNA02190277.1 ICNA0219027701 ICNA0219027705
3,3',5,5'-Tetramethylbenzidine (TMB) ≥99%, powder
3,3',5,5'-Tetramethylbenzidine (TMB)

3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620 to 650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.


Soluble in solvents 5%, Ethyl Acetate; insoluble in water. Dissolve 1,5 mg TMB in 0,1 ml of DMSO. Then add 9,9 ml of 0,1 M sodium acetate (pH 6,0). Finally, add hydrogen peroxide to a concentration of 0,01% and filter through a Whatman #1 paper filter. Prepare solutions fresh daily. Discard any remaining solution at the end of the day.


Caution: Research Use Only (RUO).

Formula: C₁₆H₂₀N₂
MW: 240,35 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00007748
CAS Number: 54827-17-7

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Specification Test Results

Identity Test Passes
Purity ≥99%
FTIR Conforms to Standard
Melting Point 165 - 170 °C

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