Tetracycline (from Streptomyces species), yellow powder
Supplier: MP Biomedicals
Tetracyline is an antibiotic substance (gram-positive, gram-negative and mycoplasma) produced by streptomyces spp.
Tetracyline inhibits gram-positive, gram-negative and mycoplasma. It is used in tetracycline controlled gene expression systems (gene switches) such as the tet-on and tet-off systems. It is also used to select tetracyline resistant colonies in cell culture.
Tetracycline binds to the 30S subunit of the ribosome at the A-site. During protein biosynthesis, the new t-RNA with the amino acid attempts to bind to the A-site of the ribosome. However, since the A-site is blocked by the tetracycline, the aminoacyl-tRNA cannot bind to it. Without the attachment of the tRNA, protein biosynthesis cannot occur. Protein biosynthesis is inhibited and cell death occurs .
Soluble in water (1,7 mg/ml), or methanol (> 20 mg/ml - clear, yellow solution).
Caution: Research Use Only (RUO).
Formula:
C₂₂H₂₄N₂O₈ MW: 444,43 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00151232 CAS Number: 60-54-8 |
Specification Test Results
Identity Test | Passes |
Potency | ≥900 μg/mg |
FTIR | Conforms to Standard |
UV/VIS: max (0.1N HCl) | 215 - 220, 266 - 271, 354 - 357 nm |
Expiration Dat | Month/Day/Yea |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...