beta-Galactosidase

Supplier: MP Biomedicals

0210493901 0210493905
ICNA0210493901EA 383 SGD
ICNA0210493901 ICNA0210493905
beta-Galactosidase
Enzymes

Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) +D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal stability: below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).


  • Substrate specificity: specifically hydrolyses β-D-galactosyl linkage


One unit catalyses the hydrolysis of 1 µmole of o-nitrophenyl-β-D-galactopyranoside per minute at 25 °C, pH 7,5.


β-Galactosidase is useful for the structural investigation of carbohydrate, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay.


β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

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