Urea ≥99%, white powder, Ultrapure

Supplier: MP Biomedicals

Synonyms: Carbonyl diamide, Carbamide, Diaminomethanone, Carbonyldiamide

0210569501 0210569505
ICNA0210569501EA 111 SGD
ICNA0210569501 ICNA0210569505
Urea ≥99%, white powder, Ultrapure
Urea

Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex.


Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.


One gram dissolves in 1 ml water, 10 ml 95% ethanol (100 mg/ml), 1 ml boiling 95% ethanol, 20 ml absolute ethanol (50 mg/ml), 6 ml methanol (166 mg/ml), 2 ml glycerol (500 mg/ml); soluble in concentrated hydrochloric acid; almost insoluble in chloroform, ether. Solutions can be treated to remove isocyanic acid. Acidification (100 mM HCl) can be used to drive the equilibrium to favor urea over the isocyanic acid, or a urea solution can be bulk deionised using an ion exchange resin.


Caution: Solutions of urea develop a significant concentration of reactive cyanate ions on standing. MP recommends making solutions fresh for each use.

Formula: NH₂CONH₂
MW: 60.06 g/mol
Boiling Pt: 197
Melting Pt: 130…140 °C
MDL Number: MFCD00008022
CAS Number: 57-13-6
Merck Index: 13,09935
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