Calcein, orange powder

Supplier: MP Biomedicals

Synonyms: Oftasceina, Fluorescein Complexon, Oftasceinum, 2,7-Bis[bis(carboxymethyl)aminomethyl]fluorescein, Oftasceine, 2,2',2'',2'''-(((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetic acid, Fluorexon, Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, NSC 298193

0219016701 0219016705 0219016710 0219016725
ICNA0219016701EA 82.1 SGD
ICNA0219016701 ICNA0219016705 ICNA0219016710 ICNA0219016725
Calcein, orange powder
Calcein

Calcein is an indicator for the determination of calcium by titration with EDTA. The excitation wavelength and emission wavelength are 495/515 nm.


It is suitable for fluorometric determination of calcium. It does not fluoresce in strong alkaline solution, but an intense fluorescence appears in the presence of alkaline earths, especially calcium. The yellow green fluorescence disappears instantly with an addition of EDTA. Also in acidic solution, the yellow green fluorescence of Calcein disappears with an addition of copper, zinc and mercury.


Calcein is used to determine the integrity and stability of free and sol-gel entrapped liposomes as well as to measure LLO pore formation, by entrapment inside the lumen of the liposomes. To study ultrasound induced drug uptake by the use of a fluorescent reporter molecule . To quantify the cellular uptake of low molecular weight fluorescent molecules due to transient enhancements of membrane permeability .


Practically insoluble in water, easily soluble in alkaline solutions to give an orange red solution.

Formula: C₃₀H₂₆N₂O₁₃
MW: 622,53 g/mol
Boiling Pt: 200 °C (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00005049
CAS Number: 1461-15-0

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