4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride), yellow powder
Supplier: MP Biomedicals
Synonyms:
2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride, DAPI.2HCl, 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride, DAPI 2HCl
4',6-Diamidino-2-phenylindole Dihydrochloride is a cell permeable fluorescent minor groove-binding probe for DNA.
- DAPI: excitation: 340 nm; emission: 488 nm (Fluorescence).
It binds to double-stranded DNA by forming a stable complex which fluoresce. DAPI selectively forms complexes with A-T, A-U and I-C base pairs (preferably A-T rich DNA), having one molecule of dye for every 3 base-pairs. DAPI in aqueous solution has two absorbance maxima, at 349 nm and at 263 nm. It can be used as a vital dye to stain nuclei in mature living pollen grains in conjuction with fluroescein diacetate.
Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Slightly soluble in water (10 mg/mL - clear, yellow to yellow-green solution - heat or sonication may be required).
Formula:
C₁₆H₁₅N₅·2HCl MW: 350,25 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00012681 CAS Number: 28718-90-3 |
Specification Test Results
Identity Test | Passes |
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