HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥98%, white powder

Supplier: MP Biomedicals

0210192625 0210192650 0210192680 0210192683 0210192690 0210192691 0210192694
ICNA0210192625EA 92.1 SGD
ICNA0210192625 ICNA0210192650 ICNA0210192680 ICNA0210192683 ICNA0210192690 ICNA0210192691 ICNA0210192694
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥98%, white powder
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers.


HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.


It is useful in the pH range 6,8-8,2.


Soluble in water giving clear, colourless solution (10% aq soln).


Caution: Store Desiccated.

Formula: C₈H₁₈N₂O₄S
Melting Pt: 234 °C (453 °F) (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Identity Test Passes
Purity ≥98%
OD250 (10% aq soln) <0.2
OD260 (10% aq soln) <0.1
OD280 (10% aq soln) <0.1
pH (1% aq soln) 4.0-5.5
Moisture <1.0%
Solubility (10% aq soln) Clear, Colorless Solution

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