3,3',5,5'-Tetramethylbenzidine (TMB), liquid substrate

Supplier: MP Biomedicals
Warning

Synonyms: TMB, 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine

0215234650 0215234680 0215234691
ICNA0215234650EA 191 SGD
ICNA0215234650 ICNA0215234680 ICNA0215234691
3,3',5,5'-Tetramethylbenzidine (TMB), liquid substrate
3,3',5,5'-Tetramethylbenzidine (TMB)

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results


  • Ready-to-Use, convenient
  • Reproducible Results
  • Consistent Performance and Sensitivity
  • Stabilized chromogen solution
  • For ELISA procedures and peroxidase and pseudo-peroxidase reactions
  • Stable up to 24 months
  • Non toxic.


Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).


Caution: Research Use Only (RUO).

Formula: C₁₆H₂₀N₂
MW: 240,35 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00007748
CAS Number: 54827-17-7

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Specification Test Results

Identity Test Passes
Functionality Test Passes
pH 3.1 ± 0.5
Expiration Date Month/Day/Year

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