HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) ≥99%, white powder for molecular biology

Supplier: MP Biomedicals

Synonyms: HEPES free acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid

0219482725 0219482780 0219482790
ICNA0219482725EA 113 SGD
ICNA0219482725 ICNA0219482780 ICNA0219482790
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) ≥99%, white powder for molecular biology
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES is a zwitterionic organic chemical buffering agent.
HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.


  • Molecular Biology Reagent Grade
  • Presentation: White Powder
  • pKa: 7.5 (25 °C) (Lit.)
  • Solutions may be autoclaved under standard conditions

Formula: C₈H₁₈N₂O₄S
MW: 238,31 g/mol
Melting Pt: 234 °C (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Identity Test Passes
Purity ≥99%
OD250 (10% aq soln) <0.2
OD260 (10% aq soln) <0.1
OD280 (10% aq soln) <0.1
pH (1% aq soln) 4.0-5.5
Moisture <1.0%
Solubility (10% aq soln) Clear, Colorless Solution

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