Tricine ≥99%

Supplier: MP Biomedicals

Synonyms: 2-((1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl)amino)acetic acid, Tricine buffer, N-(Tris(hydroxymethyl)methyl)glycine

04807410 04807413 04807416 04807420
ICNA04807410EA 132 SGD
ICNA04807410 ICNA04807413 ICNA04807416 ICNA04807420
Tricine ≥99%
Tricine

A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.


Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.


Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.


Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations. For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.

Formula: (HOCH₂)₃CNHCH₂CO₂H
MW: 179,17 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00004277
CAS Number: 5704-04-1

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