Dimethyl sulphoxide for molecular biology

Supplier: MP Biomedicals
Warning

Synonyms: Methanesulphinylmethane, Methyl sulphoxide, Dimethyl(oxido)sulphur, DMSO

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Dimethyl sulphoxide for molecular biology
Dimethyl sulphoxide

Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.


  • Molecular Biology Reagent Grade
  • Meets ACS specifications
  • Auto Ignition: 573 °F (Lit.)
  • Vapor Density: 2.7 (vs air) (Lit.)
  • Vapor Pressure: 0.42 mmHg ( 20 °C) (Lit.)
  • Refractive Index: n20D 1.479 (lit.)
  • Presentation: Liquid
  • Dielectric Constant: 45 (Lit.)
  • Soluble in water, methanol, acetone, ether, benzene, chloroform.

Formula: (CH₃)₂SO
MW: 78,14 g/mol
Boiling Pt: 189 °C (1013 hPa) (lit.)
Melting Pt: 16 …19 °C (lit.)
Density: 1,104 g/cm³ (20 °C) (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00002089
CAS Number: 67-68-5
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Specification Test Results

Assay ≥99.9%
Residue after Evaporation ≤0.01%
Titratable Acid ≤0.001meq/g
Water ≤0.1%

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