2,3,5-Triphenyltetrazolium chloride (TTC), white powder

Supplier: MP Biomedicals
Warning

Synonyms: Tetrazolium salt, Tetrazolium Red, 2,3,5-Triphenyl-2H-tetrazol-3-ium chloride, 2,3,5-Triphenyl-2H-tetrazolium chloride, TTC

0210312610 0210312625 0210312650 0210312680
ICNA0210312610EA 147 SGD
ICNA0210312610 ICNA0210312625 ICNA0210312650 ICNA0210312680
2,3,5-Triphenyltetrazolium chloride (TTC), white powder
2,3,5-Triphenyltetrazolium chloride

2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.
2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances


  • Presentation: White Powder
  • Soluble in ethanol, alcohol, water (10 mg/mL - clear, colorless solution) or acetone. Insoluble in ether.
  • Storage temperature: +4 °C, protect from light


2,3,5-Triphenyltetrazolium Chloride is a redox indicator. In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains as white TTC in areas of necrosis since these enzymes have been either denatured or degraded.


2,3,5-Triphenyltetrazolium Chloride is commonly used in biochemical experiments especially to indicate cellular respiration. TTC has been employed in autopsy pathology to assist identification of post-mortem myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase; while areas of potential infarctions will be more pale. Useful indicator for reducing substances

Formula: C₁₉H₁₅ClN₄
MW: 334,81 g/mol
Boiling Pt: ∼360 °C (1013 hPa)
Melting Pt: 243…250 °C (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00011963
CAS Number: 298-96-4
EINECS: 206-071-6
Merck Index: 13,09815

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Specification Test Results

Identity Test Passes
FTIR Conforms to Standard
I max (water) 246 ± 5 nm

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