Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals

0219485791 0219485794
ICNA0219485791EA 238 SGD
ICNA0219485791 ICNA0219485794
Urea ≥98%, white powder for molecular biology
Urea

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C


  • Molecular Biology Reagent Grade
  • Presentation: White Powder

Formula: NH₂CONH₂
Boiling Pt: 197 °C (1013 hPa)
Melting Pt: 132,7 °C
Density: 1,34 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00008022
CAS Number: 57-13-6
EINECS: 200-315-5
REACH: 01-2119463277-33
Merck Index: 13,09935
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Specification Test Results

Identity Test Passes
Purity >98%
FTIR Conforms to Standard
Melting Point 130 - 140°C
OD260 nm (7 M aq soln) <0.2
OD280 (7M aq soln) <0.2
pH (7 M aq soln) 7.5 - 9.5
PB <2 ppm

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