Iodoacetamide, tan powder

Supplier: MP Biomedicals
Danger

Synonyms: 2-Iodoacetamide

0210035180 0210035191 0210035105 0210035110 0210035125
ICNA0210035180EA 1250 SGD
ICNA0210035180 ICNA0210035191 ICNA0210035105 ICNA0210035110 ICNA0210035125
Iodoacetamide, tan powder
Iodoacetamide

Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.


Iodoacetamide can react with low molecular weight thiol compounds such as mercaptoethanol and glutathione. The reaction with glutathione is utilized in a titration method for the determination of sulfhydryl groups in different compounds. The alkylation reaction forms stable protein derivatives which will remain intact during further study of the protein.


Iodoacetamide is useful in peptide sequencing and as an irreversible enzyme inhibitor. Some enzymes iodoacetamide will inhibit include (but is not limited to): Alcohol dehydrogenase, Alkaline phosphatase (calf intestinal), beta-Amylase, Cathepsin B, Galactose oxidase, Heme oxygenase, L-Lysine decarboxylase. It will irreversibly inhibit the dephosphorylation of both epidermal growth factor receptor and platelet-derived growth factor receptor by alkylation at the active center of one or several protein tyrosine phosphatases in cell lines. Iodoacetamide will also irreversibly inhibit many cysteine proteases. Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.


Soluble in water (recommended stock solution is 10-100 mM). All solutions should be prepared fresh for each use since solutions are very light sensitive.

Formula: ICH₂CONH₂
MW: 184,96 g/mol
Boiling Pt: 266 °C (1013 hPa)
Melting Pt: 91…94 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00008028
CAS Number: 144-48-9
EINECS: 205-630-1
UN: 2811
ADR: 6.1,III

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