Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, Ultrapure

Supplier: VWR Chemicals
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Synonyms: 2-Amino-2-(hydroxymethyl)propane-1,3-diol(tris), Tromethamine, Tris(hydroxymethyl)methylamine, 2-Amino-2-(hydroxymethyl)propane-1,3-diol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trizma(R) base, TRIS base, THAM

VWRC0497-1KGEA 121 SGD
VWRC0497-1KG VWRC0497-5KG VWRC0497-500G VWRC0497-10KG VWRC0497-12KG VWRC0497-50KG
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, Ultrapure
Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Reagent for stabilisation of buffer formulations and electrophoresis of biological molecules.


Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes or biological membranes (penetration, solubilisation, adsorption on surface, etc.), have known complex forming tendency with metal ions, be non toxic and have a very low U.V. absorption at wavelength >260 nm.

Formula: NH₂C(CH₂OH)₃
MW: 121,14 g/mol
Boiling Pt: 288 °C
Melting Pt: 169 °C
Density: 1,328 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00004679
CAS Number: 77-86-1
EINECS: 201-064-4
Merck Index: 13,09842
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Specification Test Results

Abs.@280 nm (1 M, Water) <0.05
Abs.@290 nm (40%, Water) ≤0.2
Abs.@400 nm (40%, Water) ≤0.05
Arsenic ≤0.0005%
Copper ≤0.0001%
Insolubles ≤0.005%
Iron ≤0.0001%
Lead ≤0.0001%
Magnesium ≤0.0005%
Melting Point 168 – 172 °C
Moisture (KF) ≤1%
pH (5%, Water) @25 °C 10 - 11.5
Purity ≥99.9%
Solubility (10%, Water) Pass

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