Dithiothreitol (DTT, Cleland's reagent) ≥99.4% for biotechnology
Supplier: VWR Chemicals
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Warning
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Synonyms:
cis-2,3-Dihydroxy-1,4-butanedithiol, (R*,R*)-1,4-Dimercapto-2,3-butandiol, DTT, DL-1,4-Dithiothreitol
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Formula:
C₄H₁₀O₂S₂ MW: 154,25 g/mol Boiling Pt: < 125 °C (1013 hPa) Melting Pt: 42…44 °C Flash Pt: >110 °C (open cup) Storage Temperature: Refrigerator |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 |
Specification Test Results
Abs.@283 nm (0.02 M, Water) | ≤0.05 |
DNase | none detected |
Loss on Drying | <0.5% |
Melting Point | 39 – 43 °C |
Oxidised DTT | <0.5% |
Protease | none detected |
Purity (-SH Content) | >99.4% |
RNase | none detected |
Solubility (5%, Water) | pass |
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