HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, high purity
Supplier: VWR Chemicals
Synonyms:
HEPES free acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
HEPES is a general purpose buffer available for biological research.
Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation and CO₂ fixation. Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates. Recommended buffer for glutamate binding assays, preventing binding to non receptor materials.
Formula:
C₈H₁₈N₂O₄S MW: 238,31 g/mol Melting Pt: 234 °C Density: 1,448 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 EINECS: 230-907-9 Merck Index: 13,04672 |
Specification Test Results
DNase | none detected |
Heavy Metals (as Pb) | ≤0.0005% |
Iron | ≤0.0005% |
pKa @20 °C | 7.45 – 7.65 |
Protease | none detected |
Purity (Dry Basis) | >99% |
Residue on Ignition | <0.2% |
RNase | none detected |
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