Hemin chloride (from porcine), black powder

Supplier: MP Biomedicals

Synonyms: Chlorohemin, 1,3,5,8-Tetramethyl-2,4-divinylporphine-6,7-dipropionic acid ferrichloride, Chloroprotoporphyrin IX iron(III), Haemin, Hemin, Ferriprotoporphyrin IX chloride

02198820.2 0219882001 0219882010
ICNA02198820.2EA 88.7 SGD
ICNA02198820.2 ICNA0219882001 ICNA0219882010
Hemin chloride (from porcine), black powder
Hemin chloride

Hemin is an iron-containing porphyrin.It is a Protoporphyrin IX containing a ferric iron ion (Heme B) with a chloride ligand.


Hemin is used in the management of porphyria attacks, particularly in acute intermittent porphyria.It may be used to study the expression and repression of heme oxygenase 1 (HO-1) as well as the activity of HO-1.
Hemin is used for the following applications:
• To study bacterial strains and growth conditions
• Used in short-term colony assays (the culture medium consists of hemin along with other components)
• Cell transfections
• It may be used in β-hematin formation assay for analyzing the inhibitory activity of MMV (Medicines for Malaria Venture) Malaria Box compounds against the formation of β-hematin.


Typically prepared by heating a hemoglobin solution with acetic acid and sodium chloride.


Freely soluble in dilute ammonia water, also soluble in 1.4 M sodium hydroxide (254 mg/mL - dark green solution) with hematin formation, i.e., the chlorine is displaced by an OH group. Practically insoluble in carbonate solutions, dilute acid solutions. Soluble in strong organic bases such as trimethylamine, p-toluidine, dimethylaniline. Soluble in concentrated H2SO4 with loss of Fe. Sparingly soluble in 70-80% alcohol. Practically insoluble but stable in water.


Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders. Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae.


Caution: Store at +4°C, protect from light.

Formula: C₃₄H₃₂ClFeN₄O₄
MW: 651,95 g/mol
Boiling Pt: 2735 °C (1013 hPa) (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00010726
CAS Number: 16009-13-5

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Specification Test Results

Identity Test Passes
Source Porcine

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