VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix

Supplier: VWR Chemicals

VWRC733-2567EA 326 SGD
VWRC733-2567 VWRC733-2562 VWRC733-2561 VWRC733-2565
VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix
Nucleic Acid Reagents End-point PCR Enzymes and Kits

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.


The GC-Rich Template kit is specifically designed for difficult GC-rich sequences. Combined with TEMPase, GC buffers I and II promote excellent amplification. The kit is designed for initial testing before using one of the GC-TEMPase 2X Master Mixes.


Ready to use GC TEMPase 2× Master Mixes I and II are designed for amplification of GC-rich sequences.

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SPECIFICATIONS

Description GC-rich DNA Target Kit
TEMPase HotStart DNA Polymerase 5U/µl 500 units
GC TEMPase 2X Master Mix I
1.5 mM MgCl2 2500 reactions
GC TEMPase 2X Master Mix I
1.5 mM MgCl2 500 reactions
GC TEMPase 2X Master Mix II
1.5 mM MgCl2 2500 reactions
Environmentally Preferable
Format Enzyme with/without buffer Mastermix incl. dNTPs Mastermix incl. dNTPs Mastermix incl. dNTPs
Features GC-rich GC-rich GC-rich
Hot start Chemically modified Chemically modified Chemically modified Chemically modified
Concentration 5 U/µl 2x 2x 2x
Units 500 2500 r Mix 2500 500 r Mix 500 2500 r Mix 2500
Size 1 x 0.1 ml + 3 x 1.5 ml 50 x 1.25 ml 10 x 1.25 ml 50 x 1.25 ml
Buffer composition 4x GC Buffer I, 4 x GC Buffer II and 25 mM MgCl2 Based on GC buffer I Based on GC buffer I Based on GC buffer II
High fidelity No No No No
Amplicon size ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard
Exonuclease activity 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A
Storage conditions – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Application Multiplex Standard amplification,GC-rich template Standard amplification,GC-rich template Standard amplification,GC-rich template

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