Phosphate Buffered Salts, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7,2 to 7,6).
- Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells
- DPBS is used to irrigate, wash, and dilute mammalian cells
- Phosphate buffering maintains the pH in the physiological range
- Calcium and magnesium facilitate cell binding and clumping
- DPBS without these ions can be used to wash and rinse suspended cells
Inorganic Salts: Potassium Chloride [KCl]: 200,0000 mg/L; Potassium Phosphate Monobasic [KH₂PO₄]: 200,0000 mg/L; Sodium Chloride [NaCl]: 8000,0000 mg/L; Sodium Phosphate Dibasic [Na₂HPO₄]: 1150,0000 mg/L
Moisture content: <1,5%
Solubility: Dissolve one packet of powder in 1 L of distilled water. The pH will be in the range of 7,3 to 7,5. If desired, add sodium bicarbonate. The pH can be adjusted as desired using 1 N HCl or 1 N NaOH. Sterile filter, if desired. Store liquid at 2 to 8 °C.
pH: 7,4 ±0,1
Osmolality: 279 ±15 mM/kg
White powder
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