5-Methylphenazinium methyl sulphate, dark yellow powder
Supplier: MP Biomedicals
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Warning
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Synonyms:
PMS, 5-Methylphenazin-5-ium methyl sulphate, Phenazine methosulphate, Phenazine methylsulphate
Phenazine methosulfate is an electron acceptor and carrier in enzyme systems. The oxidised form is yellow and the reduced form is colourless.
Phenazine methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidised by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.
PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulphhydryl groups including those of enzymes.
Soluble in water(800 mg/4 ml), ethanol,methanol (5% w/v); insoluble in benzene, diethyl ether.
Formula:
C₁₄H₁₄N₂O₄S MW: 306,34 g/mol Melting Pt: 198 °C Storage Temperature: Freezer |
MDL Number:
MFCD00011923 CAS Number: 299-11-6 EINECS: 206-072-1 Merck Index: 12,06185 |
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