5-Methylphenazinium methyl sulphate, dark yellow powder

Supplier: MP Biomedicals
Warning

Synonyms: PMS, 5-Methylphenazin-5-ium methyl sulphate, Phenazine methosulphate, Phenazine methylsulphate

0210095510 0210095501 0210095505
ICNA0210095510EA 596 SGD
ICNA0210095510 ICNA0210095501 ICNA0210095505
5-Methylphenazinium methyl sulphate, dark yellow powder
5-Methylphenazinium methyl sulphate

Phenazine methosulfate is an electron acceptor and carrier in enzyme systems. The oxidised form is yellow and the reduced form is colourless.


Phenazine methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidised by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.


PMS is reduced by flavoproteins such as succinic dehydrogenase. PMS is reduced non-enzymatically by NADH and NADPH. PMS is also reduced by dithionite, sodium borohydride, ascorbic acid, reduced ubiquinones, and reduced vitamin K. PMS reacts with sulphhydryl groups including those of enzymes.


Soluble in water(800 mg/4 ml), ethanol,methanol (5% w/v); insoluble in benzene, diethyl ether.

Formula: C₁₄H₁₄N₂O₄S
MW: 306,34 g/mol
Melting Pt: 198 °C
Storage Temperature: Freezer
MDL Number: MFCD00011923
CAS Number: 299-11-6
EINECS: 206-072-1
Merck Index: 12,06185

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