Nitro blue tetrazolium chloride (NBT), yellow powder
Supplier: MP Biomedicals
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Danger
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Synonyms:
5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride
p-Nitroblue Tetrazolium Chloride is a substrate for dehydrogenases and other oxidases and precipitating agent for fibrinogen.
It is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
It is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Slightly (10 mg/ml) soluble in water.
Formula:
C₄₀H₃₀Cl₂N₁₀O₆ MW: 817,65 g/mol Melting Pt: 189…192 °C (lit.) Storage Temperature: Refrigerator |
MDL Number:
MFCD00012159 CAS Number: 298-83-9 EINECS: 206-067-4 |
Specification Test Results
identity Test | Passes |
l max (MeOH) | 255 ± 3 nm |
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