4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP), powder
Supplier: MP Biomedicals
Synonyms:
4-Nitrophenyl phosphate disodium salt hexahydrate, Phosphatase substrate, p-Nitrophenyl phosphate disodium salt hexahydrate, Phosphoric acid 4-nitrophenyl ester disodium salt hexahydrate, 4-Nitrophenyl phosphate disodium hexahydrate, pNPP disodium salt hexahydrate, di-Sodium 4-nitrophenyl phosphate hexahydrate
p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase.
Typically a pNPP concentration of 1 mg/ml is used
Soluble in water.
Formula:
C₆H₄NNa₂O₆P·6H₂O MW: 371,144 g/mol Melting Pt: >300 °C (lit.) Storage Temperature: Freezer |
MDL Number:
MFCD00066288 CAS Number: 333338-18-4 EINECS: 224-246-5 |
Specification Test Results
Identity Test | Passes |
Water Content | 27 - 31% |
Free p-Nitrophenol | ≤0,005% |
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