Coomassie brilliant blue G-250, dark reddish purple powder C.I. 42655

Supplier: MP Biomedicals

Synonyms: Brilliant blue G-250, Serva Blue G, Coomassie Blue G 250, Xylene Brilliant Cyanine G, Brillant blue G, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, C.I. 42655, Acid Blue 90, Sodium 3-(((4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl(3-sulphonatobenzyl)iminio)-2-methylcyclohexa-2,5-dien-1-ylidene)methyl)-3-methylphenyl)(ethyl)amino)methyl)benzenesulphonate, Brilliant indocyanine G, Brilliant Blue G

0219034380 0219034305 0219034325
ICNA0219034380EA 828 SGD
ICNA0219034380 ICNA0219034305 ICNA0219034325
Coomassie brilliant blue G-250, dark reddish purple powder C.I. 42655
Coomassie brilliant blue G-250

Coomassie Brilliant Blue G-250 is a protein stain in electrophoresis. Brilliant Blue G has been used in the Bradford dye-binding protein assay.


A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents. It is a P2X7 purinergic receptor antagonist.


A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents. It is a P2X7 purinergic receptor antagonist.


Soluble in hot water (50 mg/ml), ethanol (40 mg/ml), methanol and 10% trichloroacetic acid. Soluble in water (1 mg/ml) yielding dark Blue Solution.

Formula: C₄₇H₄₈N₃NaO₇S₂
MW: 854,04 g/mol
Density: >1 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00078482
CAS Number: 6104-58-1
EINECS: 228-058-4

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Specification Test Results

Identity Test Passes
UV/VIS: l max (methanol) 608 ± 3 nm

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