Coomassie brilliant blue G-250, dark reddish purple powder C.I. 42655
Supplier: MP Biomedicals
Synonyms:
Brilliant blue G-250, Serva Blue G, Coomassie Blue G 250, Xylene Brilliant Cyanine G, Brillant blue G, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt, C.I. 42655, Acid Blue 90, Sodium 3-(((4-((4-((4-ethoxyphenyl)amino)phenyl)(4-(ethyl(3-sulphonatobenzyl)iminio)-2-methylcyclohexa-2,5-dien-1-ylidene)methyl)-3-methylphenyl)(ethyl)amino)methyl)benzenesulphonate, Brilliant indocyanine G, Brilliant Blue G
Coomassie Brilliant Blue G-250 is a protein stain in electrophoresis. Brilliant Blue G has been used in the Bradford dye-binding protein assay.
A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents. It is a P2X7 purinergic receptor antagonist.
A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents. It is a P2X7 purinergic receptor antagonist.
Soluble in hot water (50 mg/ml), ethanol (40 mg/ml), methanol and 10% trichloroacetic acid. Soluble in water (1 mg/ml) yielding dark Blue Solution.
Formula:
C₄₇H₄₈N₃NaO₇S₂ MW: 854,04 g/mol Density: >1 g/cm³ (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00078482 CAS Number: 6104-58-1 EINECS: 228-058-4 |
Specification Test Results
Identity Test | Passes |
UV/VIS: l max (methanol) | 608 ± 3 nm |
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