HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, Ultrapure Bioreagent for liquid chromatography, for molecular biology, J.T.Baker®

Supplier: Avantor
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BAKR4018-04EA 215 SGD
BAKR4018-04
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99%, Ultrapure Bioreagent for liquid chromatography, for molecular biology, J.T.Baker®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

Free acid for liquid chromatography and molecular biology buffers. Lot analysis on label.


  • Lot analysis on label

Formula: C₈H₁₈N₂O₄S
Melting Pt: 210…215 °C
Density: 1,448 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
Merck Index: 13,04672
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Specification Test Results

For Laboratory,Research,or Manufacturing Use ≥99%
Assay (C₈H₁₈N₂O₄S) ≥99 %
Appearance (fine, white, crystalline powder) Passes Test
Sodium (USP Id.) Passes Test
DNase Activity (None Detected) Passes Test
RNase Activity Passes Test
Protease Activity Passes Test
Absorbance of a 0.1M Solution (1-cm path vs water) at - 260 nm ≤0.050
Absorbance of a 0.1M Solution (1-cm path vs water) at - 280 nm ≤0.050
pH of 5% Solution at 25°C 5.0 - 6.5
Ash (sulfated) ≤0.2 %
Arsenic and Antimony (as As) ≤0.050 ppm
Trace Impurities - ACS - Heavy Metals (as Pb) ≤5 ppm
Trace Impurities - Iron (Fe) ≤5 ppm
pK^a at 20°C, For Information Only
QC Supervisor or equivalent to check supplier's COA to ensure proper chemical form. Passes Test

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