5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-Na) ≥99%, off-white powder
Supplier: MP Biomedicals
Synonyms:
5-Bromo-4-chloro-1H-indol-3-yl phosphate sodium, X-phosphate disodium salt, Sodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
A chromogenic substrate for alkaline phosphatase.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Disodium salt is soluble in water (50 mg/ml); insoluble in dimethylformamide.
Formula:
C₈H₄BrClNNa₂O₄P MW: 370,43 g/mol Melting Pt: >300 °C (lit.) Storage Temperature: Freezer |
MDL Number:
MFCD00036757 CAS Number: 102185-33-1 |
Specification Test Results
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Reference |
Loss on Drying | ≤10% |
UV/VIS: I max (water) | 291 ± 3nm |
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