You Searched For: Extraction+Buffers

Catalog Number: (VWRCM334-100ML)

Supplier: VWR Chemicals

Description: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

UOM: 1 * 100 mL

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Supplier: Thermo Scientific

Description: RBC lysis buffer for human, Thermo Fisher Scientific

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Supplier: Avantor

Description: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

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Catalog Number: (ALFAJ62885.AE)

Supplier: Thermo Scientific

Supplier Article Number:: J62885.AE

Description: RIPA buffer with Triton® X-100 (5X), Thermo Fisher Scientific

UOM: 1 * 100 mL

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Supplier: Thermo Scientific

Description: RBC lysis buffer for mouse, Thermo Fisher Scientific

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Catalog Number: (ALFAJ62939.AK)

Supplier: Thermo Scientific

Supplier Article Number:: J62939.AK

Description: Phosphate buffered RIPA with glycerol (2X), Thermo Fisher Scientific

UOM: 1 * 250 mL

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Supplier: Avantor

Description: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Catalog Number: (ALFAJ60720.AP)

Supplier: Thermo Scientific

Supplier Article Number:: J60720.AP

Description: Thermo Fisher Scientific

UOM: 1 * 500 mL

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Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Catalog Number: (ALFAJ62745.XE)

Supplier: Thermo Scientific

Supplier Article Number:: J62745.XE

Description: Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

UOM: 1 * 125 mL

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Catalog Number: (VWRC0883-100ML)

Supplier: VWR Chemicals

Description: Phenol:Chloroform pH 6.7/8.0 is premixed with isoamyl alcohol (25:24:1). A phenol:chloroform mixture is ideal for the extraction of protein from DNA preparations. Packaged at pH 6.7, this product is accompanied by a separate alkaline buffer which allows the researcher to increase the pH to 8.0. Doubly distilled, high purity phenol packaged under nitrogen. Safer, easier alternative to crystalline phenol.

UOM: 1 * 100 mL

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Supplier: OMEGA BIO-TEK

Description: Elution buffer, Omega Bio-Tek

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Supplier: OMEGA BIO-TEK

Description: SP2 buffer, Omega Bio-Tek

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Supplier: OMEGA BIO-TEK

Description: Modified from the E.Z.N.A.® Plant RNA kit, the E.Z.N.A.® Fungal RNA kit uses an improved buffer system that is more efficient for fungal samples. This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. Rather, the method involves a simple and rapid precipitation step for removal of much of the polysaccharides and phenolic compounds commonly found in fungal tissues. In combination with HiBind® RNA spin columns, this method permits purification of high quality RNA from as much as 200 mg tissue. The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. Purified RNA can be used for many downstream applications such as RT-PCR, northern blotting, Poly(A)+ mRNA purification, and differential display.

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Supplier: OMEGA BIO-TEK

Description: E.Z.N.A.® MicroElute® Genomic DNA kit provides a rapid and easy method for the isolation of genomic DNA from small sample volumes and sizes for consistent PCR and analytical test results. Using the reversible binding properties of a silica-based material, the speed of mini-column spin technology, and specifically formulated buffers, the system allows the purification of genomic DNA up to 60 kb. Samples are first lysed under denaturing conditions and then applied to the HiBind MicroElute® Column to bind DNA, while cellular debris, haemoglobin, and other proteins are effectively washed away. High quality DNA can be eluted with 10 to 15 µl sterile deionised water or elution buffer from the MicroElute® column.

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Supplier: PanReac AppliChem

Description: Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.