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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-VWRC1B1301-KIT

VWR® Protein Precipitation Kit

Catalog Number: (VWRC1B1301-KIT)
Supplier: VWR Chemicals
Description: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
UOM: 1 * 1 KIT
Product Image-VWRCM172-1L

VWR®, Bradford protein assay, proteomics grade

Catalog Number: (VWRCM172-1L)
Supplier: VWR Chemicals
Description: The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.
UOM: 1 * 1 L

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Product Image-BSBTAR1110

Micro BCA protein assay kit

Catalog Number: (BSBTAR1110)
Supplier: BosterBio
Supplier Article Number:: AR1110
Description: The Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve.
UOM: 1 * 1 KIT
Product Image-GENO786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Catalog Number: (GENO786-005)
Supplier: G-Biosciences
Supplier Article Number:: 786-005
Description: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
UOM: 1 * 1 KIT
Image Unavailable-ICNA0210072610

Histone, from calf thymus

Supplier: MP Biomedicals
Description: Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

Product Image-SOLUB-9007-105K

Biotinylation kits, ChromaLink™ Biotin Antibody Labelling kit

Catalog Number: (SOLUB-9007-105K)
Supplier: TRILINK BIOTECHNOLOGIES
Supplier Article Number:: B-9007-105K
Description: ChromaLink™ Biotin Protein Labelling kit offers a complete solution for the quantitative biotinylation of proteins from 20 kDa to 200 kDa and from 25 µg to 1 mg. The kit incorporates traceable biotin on proteins and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per protein molecule.
UOM: 1 * 1 KIT

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Product Image-GEHE80-6483-56

2-D Quant kit

Catalog Number: (GEHE80-6483-56)
Supplier: Cytiva
Supplier Article Number:: 80-6483-56
Description: 2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.
UOM: 1 * 1 KIT

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Product Image-PIER23209

Pierce™ BCA™ Protein assay kits and reagents

Supplier: Thermo Fisher Scientific
Description: Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

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Product Image-PIER23240

Modified Lowry protein assay kit, Pierce™

Catalog Number: (PIER23240)
Supplier: Thermo Fisher Scientific
Supplier Article Number:: 23240
Description: The Modified Lowry Protein Assay Kit is the preferred colorimetric assay for quantifying total protein concentration.
UOM: 1 * 1 KIT

MSDS Certificates


Product Image-PIER23236

Pierce™ Coomassie Plus (Bradford) Protein Assay Kit

Catalog Number: (PIER23236)
Supplier: Thermo Fisher Scientific
Supplier Article Number:: 23236
Description: The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.
UOM: 1 * 1 KIT

MSDS


Image Unavailable-PIER22030

Biotin-fluorescein conjugate, Pierce™

Catalog Number: (PIER22030)
Supplier: Thermo Fisher Scientific
Supplier Article Number:: 22030
Description: Biotin-Fluorescein is a heterobifunctional reagent containing a biotin moiety and a fluorescein group separated by a long-chain hydrophilic polyethylene glycol (PEG) spacer arm. Biotin-Fluorescein is used to label biotin binding protein molecules including avidin and streptavidin, which each have four biotin-binding sites. The resulting fluorescent biotin-binding protein can then amplify a signal generated in assays that use avidin-biotin detection systems.
UOM: 1 * 5 mg
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +65 6505 0760.
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