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You Searched For: Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-VWRC1B1301-KIT

VWR® Protein Precipitation Kit

Catalog Number: (VWRC1B1301-KIT)
Supplier: VWR Chemicals
Description: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
UOM: 1 * 1 KIT
Product Image-VWRCM172-1L

VWR®, Bradford protein assay, proteomics grade

Catalog Number: (VWRCM172-1L)
Supplier: VWR Chemicals
Description: The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.
UOM: 1 * 1 L

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Product Image-GENO786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Catalog Number: (GENO786-005)
Supplier: G-Biosciences
Supplier Article Number:: 786-005
Description: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
UOM: 1 * 1 KIT
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Histone, from calf thymus, MP Biomedicals

Supplier: MP Biomedicals
Description: Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

Product Image-SOLUB-9007-105K

Biotinylation kits, ChromaLink™ Biotin Antibody Labelling kit

Catalog Number: (SOLUB-9007-105K)
Supplier: TRILINK BIOTECHNOLOGIES
Supplier Article Number:: B-9007-105K
Description: ChromaLink™ Biotin Protein Labelling kit offers a complete solution for the quantitative biotinylation of proteins from 20 kDa to 200 kDa and from 25 µg to 1 mg. The kit incorporates traceable biotin on proteins and allows for direct spectrophotometric verification of biotin incorporation. A simple Å280 and Å354 measurement determines the number of biotins per protein molecule.
UOM: 1 * 1 KIT

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at +65 6505 0760.
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