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dNTP mix

Catalog Number: (QUNT95062-01K)

Supplier: Quantabio

Supplier Article Number:: 95062-01K

Description: The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

UOM: 1 * 1.000 µl

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AccuStart™ II PCR SuperMix

Supplier: Quantabio

Description: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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AccuStart™ II GelTrack PCR SuperMix

Supplier: Quantabio

Description: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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qPCR SuperMix, PerfeCTa® MultiPlex

Supplier: Quantabio

Description: This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

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Ethidium bromide

Catalog Number: (ICNA04802511)

Supplier: MP Biomedicals

Supplier Article Number:: 04802511

Description: Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

UOM: 1 * 10 mL

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VWR®, Phenol-free total RNA purification kit

Catalog Number: (VWRCN788-KIT)

Supplier: VWR Chemicals

Description: The phenol-free total RNA purification kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses.

UOM: 1 * 1 KIT

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Peqlab peqGOLD, RNA Isolation, Total RNA Kit

Supplier: VWR Chemicals

Description: Easy to use method for isolation up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues <1×10⁷ cells or 30 mg tissue.

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VWR®, dTTP solution

Catalog Number: (VWRCE542-40UM)

Supplier: VWR Chemicals

Description: Deoxyribonucleotides are the foundation of DNA and it takes thousands of these nucleotides to make up DNA.

UOM: 1 * 40 µmol

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VWR® ExoCleanUp FAST, One-step PCR Clean-up

Supplier: VWR Chemicals

Description: VWR® ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).

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Formamide ≥99.5% (by GC), Ultrapure Bioreagent for denaturing nucleic acids prior to analysis, J.T.Baker®

Supplier: Avantor

Description: For denaturing nucleic acids prior to analysis.

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VWR®, Taq Plus 2X master mixes

Supplier: VWR Chemicals

Description: VWR® Taq Plus is an optimised format of Taq DNA polymerase master mix and, therefore, is a good alternative to Taq DNA polymerase and Taq DNA polymerase master mix.

DNAzol® DNA isolation reagent

Supplier: Thermo Scientific

Description: Applications: Complete and ready to use reagent for the isolation of genomic DNA from solid and liquid samples of animal and plant origin

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