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Supplier: MP Biomedicals
Description: RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Supplier: MP Biomedicals
Description: Wesson's Salt mixture is designed for the insect diet mixture.

Supplier: MP Biomedicals
Description: Tocopherol acetate has properties similar but not identical to α-tocopherol.

Supplier: MP Biomedicals
Description: N-dodecyl-β-D-maltoside is used as a non-ionic detergent for stabilization and activation of enzymes and for membrane research.

Supplier: MP Biomedicals
Description: Aquaphenol™ is a solution of double-distilled phenol saturated with ultra-pure water (approx 30%). It does not contain antioxidant or additives. Aquaphenol™ has an acid pH and can be used directly for RNA extraction. For DNA extraction, pH can be quickly adjusted to 7,5 or 8 using pH maker solutions and protocols included.

Supplier: MP Biomedicals
Description: Sulphanilic acid is a reagent for chromatographic detection of histidine, and intermediate for synthesis of dyes and pharmaceuticals.

Supplier: MP Biomedicals
Description: An inhibitor of Xanthine Oxidase which decreases uric acid production; also inhibits de novo pyrimidine biosynthesis.

Supplier: MP Biomedicals
Description: Ammonium bicarbonate is used in the manufacture of porous plastics, ceramics, dyes, and pigments, fire extinguishers. It has been used in the preparation and isolation of neoglycoprotein conjugates.
Supplier: MP Biomedicals
Description: L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine.

Supplier: MP Biomedicals
Description: Deoxycytidine is a deoxyribonucleoside, a component of deoxyribonucleic acid. It is similar to the ribonucleoside cytidine, but with one hydroxyl group removed from the 2' position.

Supplier: MP Biomedicals
Description: Alkaline phosphatase is a phosphomonoesterase, used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.
Supplier: MP Biomedicals
Description: L-Glutamic Acid is a non-essential amino acid for human development; referred to as an excitatory amino acid (EAA) due to its role in neurotransmission. Acts as a neurotransmitter at fast synapses; agonist at kainate, NMDA and quisqualate receptors. L-Glutamic acid is one of the two amino acids that contains a carboxylic acid group in its side chains.
Supplier: MP Biomedicals
Description: Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutamyl amino acids and reductive cleavage of disulphide bonds.

Supplier: MP Biomedicals
Description: Deoxyribonucleic acid sodium salt has been used in a study to evaluate the cytotoxicity of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. Deoxyribonucleic acid sodium salt has also been used in a study to develop a simple method for extracting DNA from Cryptosporidium oocysts.

Catalog Number: (ICNA04802511)
Supplier: MP Biomedicals
Supplier Article Number:: 04802511
Description: Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
UOM: 1 * 10 mL


Supplier: MP Biomedicals
Description: Dexamethasone is an anti-inflammatory glucocorticoid that induces the production of phospholipase A2 inhibitory protein (lipocortin).

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