How to Optimize your Charge Variant Analysis

Taking charge of variant analysis

The analysis of charged variants is a regulatory requirement for bio-therapeutic proteins. Ion exchange chromatography is useful in the analysis of enzymatic post-translational modifications and chemical modifications that can occur on a bio-therapeutic.

Understanding column selection (WCX vs SCX) and buffer optimization (salt vs pH gradients) is key to developing a robust, high resolution, and sensitive assay. Join us to hear about optimising ion exchange method development, best practices, and the latest innovations of coupling charged variant analysis to mass spectrometry (CVA-MS).