You Searched For: Protein+Expression

Catalog Number: (VWRCJ869-500G)

Supplier: VWR Chemicals

Description: Terrific broth is an enriched medium, ideal for culturing cells for nucleic acid purification and recombinant protein expression.

UOM: 1 * 500 g

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Catalog Number: (PPLU101000017)

Supplier: Polyplus-transfection

Supplier Article Number:: 101000017

Description: PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus and recombinant proteins by Transient Gene Expression (TGE) in suspension-adapted mammalian cell lines cultivated in shaker flasks, platform shakers or stirred tank bioreactors.

UOM: 1 * 1,5 mL

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Catalog Number: (TNBO80-0032-U100)

Supplier: Tonbo Biosciences

Supplier Article Number:: 80-0032-U100

Description: The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

UOM: 1 * 100 µG

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Catalog Number: (ICNA08642861)

Supplier: MP Biomedicals

Supplier Article Number:: 08642861

Description: Freund's incomplete adjuvant mixture contains mineral oil and Arlacel A.

UOM: 1 * 50 mL

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Catalog Number: (ICNA08642851)

Supplier: MP Biomedicals

Supplier Article Number:: 08642851

Description: Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of <i>Mycobacterium</i> is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.

UOM: 1 * 50 mL

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Supplier: MP Biomedicals

Description: Hemoglobin is used to study paradigm for the regulation of gene expression and cellular differentiation.

Supplier: MP Biomedicals

Description: Methotrexate is a cell cycle arresting agent with varying effects. Methotrexate has been reported to arrest the cell cycle in late G1/S thus leading to the inhibition of the synthesis of DNA, RNA, thymidylates, and proteins. The main mechanism of action is reported to involve the inhibition of enzymes involved in purine metabolism which leads to the accumulation of adenosine or the suppression of intercellular adhesion molecule expression by T cells. Additionally, this compound has been observed to inhibit DHFR.
Methotrexate is used for chemotherapy either alone or in combination with other agents. It is effective for the treatment of a number of cancers including: breast, head and neck, leukemia, lymphoma, lung, osteosarcoma, bladder, and trophoblastic neoplasms. It is also used in treatment of autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It is used to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.

Catalog Number: (ICNA113046075)

Supplier: MP Biomedicals

Supplier Article Number:: 113046075

Description: Terrific broth (TB) has been formulated for optimum growth of <i>Escherichia coli</i> for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using this broth.

UOM: 1 * 10 L

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Supplier: MP Biomedicals

Description: Terrific broth (TB) has been formulated for optimum growth of <i>Escherichia coli</i> for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using this broth.

Catalog Number: (ICNA112200200)

Supplier: MP Biomedicals

Supplier Article Number:: 112200200

Description: Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into <i>Pichia pastoris</i> for protein expression studies.

UOM: 1 * 250 Tests

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Supplier: MP Biomedicals

Description: Storage: Store at Room Temperature (15-30 °C). Store Desiccated. Store under nitrogen.
L-Cysteine is a proteinogenic amino acid incorporated into proteins as directed by the genetic code. The thiol-side chain participates in a variety of oxidation/reduction reactions within the cell. The side chain participates in the formation of β bonds that modulate the secondary and ternary structure of proteins. Cysteine is essential and limiting for the formation of glutathione, an important antioxidant, within the cell.
Cysteine has been used in pulse-chase experiments to study protein expression from an inducible promoter. L-Cysteine is used to supplement cell culture media.
NMDA glutamatergic receptor agonist.

Supplier: MP Biomedicals

Description: IPTG (isopropyl-β-thiogalactoside) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli.
IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.
The final concentration typically used in indicator plates is 0.2 mM.

Supplier: MP Biomedicals

Description: Storage: Store at -20 °C.
Brefeldin A is a fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. Produced by Penicillium brefeldianum. Blocks binding of the cytosolic coat protein b-COP and ARF to Golgi membranes mediated by protein G. Also blocks protein transportation into post-Golgi compartments. It activates the sphingomyelin cycle. Brefeldin A mediated apoptosis has been observed in human tumor cells.
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.

Catalog Number: (ICNA08670281)

Supplier: MP Biomedicals

Supplier Article Number:: 08670281

Description: This product is developed in goat using mouse IgG purified from pooled normal mouse serum as the immunogen. IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. It regulates several functions such as complement activation and phagocytosis and hence plays a pivotal role in facilitating cytological immune responses. Anti-mouse IgG (whole molecule) antibody can be used in T cell proliferation assays. It can also be used in Ouchterlony Double Diffusion and immunoelectrophoresis. Goat anti- mouse IgG (whole molecule) antibody reacts specifically with mouse IgG subclasses IgG1, IgG2a, IgG2b and IgG3. The product has also shown no reactivity to human serum proteins.

UOM: 1 * 2 mg

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Supplier: PAN-BIOTECH GMBH

Description: DMEM for SILAC is optimised for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyse protein expression by mass spectrometry (MS).

Supplier: MP Biomedicals

Description: Storage: Store at +4 °C, store under nitrogen
Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. The sulfhydryl (thiol) group (SH) of cysteine serves as a proton donor and is responsible for the biological activity of glutathione.
Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds.
Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.