You Searched For: Cell+Freezing+Media

Catalog Number: (VWRCN676-50ML)

Supplier: VWR Chemicals

Description: SeraFree cryopreservation media is ready to use freezing media for cryopreservation of adherent or suspension cultured cells. The animal-free media composition eliminates batch-to-batch variability and is optimised for cell viability and cell growth after thawing. Suitable for use in environments that prohibit the use of animal-derived products.

UOM: 1 * 50 mL

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Supplier: PAN BIOTECH

Description: Freezing medium, cryopreservation-medium for the cryoconservation of cells. The medium is based on DMEM, supplemented with a mix of fetal bovine serum and DMSO.

Catalog Number: (ICNA0920300M2)

Supplier: MP Biomedicals

Supplier Article Number:: 0920300M2

Description: Cellvation is a cryopreservation medium designed for optimal cell recovery and convenience.

UOM: 1 * 60 mL

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Supplier: VWR Chemicals

Description: Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

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Supplier: VWR Chemicals

Description: These standard supplements can be used with several culture media to prepare the complete culture media for your specific purpose. Most of these supplements are available as freeze-dried in vials and this ensures.

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Supplier: VWR Chemicals

Description: The isolation of microorganisms often requires the use of specific supplements. The supplements presented here are available in vials and bottles, and the corresponding culture medium to use with them is indicated in the table below. Usually each vial is used to supplement 500 ml of media unless otherwise stated (complete information available on the specification sheets available on request).

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Supplier: MP Biomedicals

Description: Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals.

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

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Catalog Number: (VWRU414004-284)

Supplier: VWR Collection

Supplier Article Number:: 414004-284

Description: Polycarbonate cooler, with floating inserts.

UOM: 1 * 1 items

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Supplier: Corning

Description: CoolCell® alcohol-free cell freezing containers ensure standardised controlled-rate −1 °C/minute cell freezing in a −80 °C freezer, without alcohol or any fluids.

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Supplier: VWR Chemicals

Description: RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

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Supplier: VWR Chemicals

Description: Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

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Catalog Number: (VWRC392-0421)

Supplier: VWR Chemicals

Description: Medium 199 was developed specifically for nutritional research of chicken fibroblasts. Today, 199 media are widely used for the maintenance of non transformed cells, vaccine and virus production and primary explants of epithelial cells. The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO₂ incubator results in a rapid drop in pH of the culture medium.

UOM: 1 * 500 mL

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Supplier: VWR Chemicals

Description: Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

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Catalog Number: (VWRC392-0420)

Supplier: VWR Chemicals

Description: McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

UOM: 1 * 500 mL

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Supplier: VWR Chemicals

Description: Dulbecco's Modified Eagle Medium (DMEM) is designed to preserve and maintain the growth of a broad spectrum of mammalian cell types.

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Catalog Number: (VWRC392-0419)

Supplier: VWR Chemicals

Description: Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from <i>Drosophila spp</i> and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

UOM: 1 * 500 mL

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