You Searched For: Cloning+Kits+and+Ligation

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Cloning supplement

Catalog Number: (PANBP04-95901)

Supplier: PAN-BIOTECH GMBH

Supplier Article Number:: P04-95901

Description: This supplement stored at −20 °C.

UOM: 1 * 1 mL

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Ligation Sequencing Kits

Catalog Number: (OXNTSQK-LSK109)

Supplier: Oxford Nanopore Technologies

Description: Ligation Sequencing Kits enable ligation-based, PCR-free nanopore sequencing of native DNA.

UOM: 1 * 1 KIT

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GeneClean® PCR clean-up, turbo for PCR kit

Supplier: MP Biomedicals

Description: The GeneClean® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with this kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

Agarose, Electran, high resolution for electrophoresis

Supplier: VWR Chemicals

Description: Agarose high resolution (HR) has melting, max. 90 °C, and gelling temperatures (32,5 to 38 °C), but differs from traditional low melting point molecular biology grade agaroses in the gel separation range. Using this agarose in electrophoresis allows fine and consistent resolution of nucleic acids below 1000 base pairs, which differ by only a few base pairs. The properties of Agarose HR allow consistent gel separations (analytical and preparative) and performance of <i>in-vitro</i> translation and transcription mapping, as well as <i>in-vivo</i> ligation and transformation. For analytical gel separation of DNA/RNA below 1000 base pairs, where a higher gel strength for ease of manipulation is required, we recommend the use of Agarose, high resolution, Electran (43655).

MSDS Certificates

Calf alkaline phosphatase (from intestine)

Supplier: MP Biomedicals

Description: Alkaline phosphatase is a phosphomonoesterase, used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.

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Guanosine 97.0-103.0%, off-white powder

Supplier: MP Biomedicals

Description: Guanosine is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine can be phosphorylated to become guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosphate (GDP), and guanosine triphosphate (GTP). These forms play important roles in various biochemical processes such as synthesis of nucleic acids and proteins, photosynthesis, muscle contraction and intracellular signal transduction (cGMP). When guanine is attached by its N9 nitrogen to the C1 carbon of adeoxyribose ring it is known as deoxyguanosine.
Guanosine is required for an RNA splicing reaction in mRNA, when a "self-splicing" intron removes itself from the mRNA message by cutting at both ends, re-ligating, and leaving just the exons on either side to be translated into protein. The antiviral drug aciclovir, often used in herpes treatment, is structurally similar to guanosine.

ThermoMixer® C Thermomixer

Catalog Number: (EPPE5382000.031)

Supplier: EPPENDORF

Supplier Article Number:: 5382000.031

Description: The all-in-one for heating/cooling and mixing samples and reactions. The Eppendorf® ThermoMixer C convinces users with its quick and easy-to-exchange SmartBlocks for all common laboratory vessels. There is almost no limit to the variety of applications waiting for you.

UOM: 1 * 1 items

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DNA purification kits, GeneClean® II kit

Catalog Number: (ICNA111001400)

Supplier: MP Biomedicals

Supplier Article Number:: 111001400

Description: The GeneClean® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

UOM: 1 * 1 KIT

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DNA clean up kits, GeneClean® Turbo Kits

Supplier: MP Biomedicals

Description: GeneClean® Turbo Kit is used for purification of DNA fragments of sizes 0,1 kb to 300 kb from TAE or TBE buffered agarose gels, PCR reactions and other enzymatic solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Plasmid purification, MiniPrep Express™ Matrix

Catalog Number: (ICNA112000200)

Supplier: MP Biomedicals

Supplier Article Number:: 112000200

Description: MiniPrep Express™ Matrix is designed for rapid purification of plasmid DNA. It is an ideal tool for screening hundreds of recombinant clones in record time using restriction enzyme analysis and PCR.

UOM: 1 * 1 KIT

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